目的 对自然周期体外受精/体外成熟(IVF/IVM)技术治疗卵巢低反应引起的不育症的临床效果进行初步探讨.方法 31例适应证患者接受34个自然周期IVF/IVM治疗.取卵前36 h注射人绒毛膜促性腺激素(hCG) 10,000 IU.抽吸优势卵泡和小卵泡的卵母细胞,成熟卵母细胞于2～3 h后进行卵胞浆内单精子注射(ICSI)；未成熟卵母细胞体外培养24或48 h后,成熟卵母细胞ICSI受精.得到的胚胎于取卵后第3天进行移植.结果 取消周期4个,共27例患者取卵成功30个周期.11例获得临床妊娠,9例已分娩,全部为单胎；1例妊娠中；1例宫外孕.结论 自然周期IVF/IVM对于治疗卵巢低反应不育症是一种简便有效的方法.%Objective: To explore the clinical efficiency of new alternative-natural cycle IVF combined with IVM of immature oocytes (natural cycle IVF/M) to treat infertile patients with poor responding.Methods; Thirty one women with normal ovaries underwent treatment for 34 cycles of natural cycle IVF/M. Human chorionic gonadotropin (hCG) was administered (10,000 IU) 36 hours before oocyte retrieval in a natural cycle. After aspiration of dominant follicle and small follicles, the mature oocytes were inseminated by intracytoplasmic sperm injection (ICSI) immediately. The immature oocytes were matured in vitro and inseminated by ICSI, and then the embryos transferred.Results: Four oocyte pickup cycles were cancelled. The oocytes of 27 patients were successfully retrieved in 30 cycles. Twenty seven ET cycles were completed, in which 11 patients were clinically pregnant. Nine patients had delivered. One patient was ongoing pregnancy and one patient had miscarried because of ectopic pregnancy.Conclusions: Natural cycle IVF/M may be an alternatively efficient method for infertile patients with poor responding.
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