自第一例冻融人类胚胎获得妊娠以来,胚胎冷冻已经成为辅助生殖技术中的一项常规操作.近年来,玻璃化冷冻技术成功用于冻存人类卵母细胞及胚胎.那么,玻璃化冷冻方法已经安全到可以广泛应用,甚至可以取代慢速冷冻方法了吗？实际上仍有许多问题有待解决,包括冷冻保护剂毒性、开放式载体的交叉污染、复苏胚胎发育潜能及损伤鉴定等.%Since the first report of a pregnancy following replacement of frozen-thawed human embryos, embryo cryopreservation has become a routine procedure. In recent years, vitrification method has been used successfully to cryopreserve human oocytes and embryos. Is vitrification really safe enough to be used widely, even substitute the slow freezing. Actually we should pay more attention to some problems of vitrification. It includes cryoprotectant toxicity, the cross-contamination in liquid nitrogen tanks when embryos are loaded in open carriers, the safety assessment of embryos after vitrification and many other concerns.
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