The genetic diversity of five populations of Curcuma wenyujin,a species endemic to Ruian,Yueqing Pingyang and,was analyzed using inter simple sequence repeat(ISSR). A total of 174 6 bands and 42 loci were amplified from 5 populations by 6 informative and reliable primers of 40 primers. Of them,34 loci were polymorphic, which accounted for 80.95%. Genetic diversity was revealed,Ht=0.239 5,Hs=0.1618,Dst=0.077 7 and GST=0.324 5. The study shows that there has a middle degree of genetic differentiation occurred among Curcuma wenyujin populations and a high genetic variation level beyond the populations of Curcuma wenyujin. Thus,Nm=1.041 0 means genetic was more keep in touch with each other among the population of Curcuma wenyujin.%应用ISSR标记技术对瑞安、乐清和平阳三个地区5个居群的温郁金（Curcuma wenyujin）遗传多样性进行分析，筛选出6个引物用于温郁金5个居群的50个个体ISSR-PCR扩增，共检测到42个结合位点多态位点34个，多态位点百分率是80.95%．总的基因多样度平均值为0.2395，居群内的基因多样度为0.1618，居群间的基因多样性为0.0777．基因分化系数在0.0191～0.9414之间，平均值为0.3245，其中居群间的遗传变异占总遗传变异的32.45%，居群内的遗传变异占总变异的67.55%．温郁金有着较高的遗传多样性水平，且居群内发生了较高的遗传分化．基因流系数Nm为1.0410，表明温郁金居群间的基因交流也比较多．
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