对粳稻"嘉花1号"经60Coγ诱变处理获得的稳定遗传低温敏感叶色突变体tcm11(thermo-sensitive chloroplast mutant 11)进行了表型鉴定与遗传分析.在20℃条件下,该突变体三叶期之前幼苗均表现为黄色,光合色素含量明显下降,叶绿体发育不完整,从第4叶开始逐渐转为浅黄绿色直至最后死亡.而在32 ℃条件下,其表型与野生型相比没有明显差异,具有低温敏感属性.通过对培矮64S与tcm11杂交的F2代分离群体进行遗传分析,发现该低温敏感突变体性状是受单个隐性核基因(tcm11)控制,利用图位克隆技术对tcm11进行定位,将其定位在第11号染色体的InDel分子标记ID13252与SSR分子标记MM1361之间一个约1 566 kb的区域内.这也为后续的研究奠定了基础.%A stable geneticlow temperature sensitive leaf color mutant tcm11 (thermo-sensitive chloroplast mutant11) derived from Japonicarice variety Jiahua No.1 treated by 60Co γ-rays was used in this study,whichwasidentified the phenotypic characterization and genetic analysis.Underthe condition of 20 ℃,the tcm11 mutant was showed the etiolation phenotype before 3-leaf-stage,which were greatly reduced the photosynthetic pigment contents andbroken the chloroplast structure.However,it began to turn the yellow green phenotype after 4-leaf-stageuntil it died.But under the condition of 32 ℃,its phenotype was no significant difference compared with wild type(Jiahua No.1),with low temperature sensitive attributes.Using segregatingindividuals from cross of Pei′ai 64S and tcm11,genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene.Through map-based cloning,the mutant gene (tcm11) was located to 1 566 kb region between the molecular markers ID13252 and MM1361 on rice chromosome 11,which also the follow-up research laid the foundation for in the future.
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