Objective To observe the changes of levels of TNF-α and IL-10 in plasma,lung and kidney of the acute poisoned rats induced by paraquat for exploring the mechanism of organ damaged by paraquat poisoning.Methods The rats were intraperitoneally injected with 20％ paraquat solution( 30 mg/kg )in PQ group and injected with saline in control group.The levels of tumor necrosis factorα( TNF-α )and interleukin-10( IL-10 )in plasma, lung and kidney were determined after poisoning rats for 2 h,6 h, 12 h,24 h,72 h and 120 h.Results The levels of TNF-α in plasma,lung and kidney increased gradually within 72 h in PQ group,and decreased at 120 h.TNF-α levels in plasma and lung were significantly higher at 6 h, 12 h,24 h,72 h, 120 h in PQ group than that in control group( P ＜0.05 ),and the levels of TNF-α in kidney were significantly higher at 12 h,24 h,72 h, 120 h in PQ group than that in control group ( P ＜0.05 ).IL-10 levels increased gradually within 120 h in plasma,lung,kidney in rats.At 12 h,24 h,72 h,120 h,the IL-10 levels in plasma, lung of PQ rats were statistically higher than that in control rats( P ＜ 0.05 ).The levels of IL-10 in kidney of PQ rats were significantly higher at 24 h,72 h, 120 h than that in control rats( P ＜ 0.05 ).Conclusion Paraquat poisoning can cause the damages of lung and kidney, and the damage degrees are closely related with the changes of TNF-α and IL-10 levels, which suggests that the damage may be related with the involvement of TNF-α and IL-10 in the inflammatory response and cellular immune.%目的 观察百草枯急性中毒后不同时间段引起血浆以及肺、肾组织中炎症因子TNF-α和IL-10的变化,为探讨百草枯中毒引起的脏器损害机制提供理论依据.方法 以20%百草枯溶液(30 mg/kg)制作大鼠中毒模型(PQ组),分别观察模型大鼠在造模2 h,6 h,12 h,24 h,72 h以及120 h其血浆以及肺、肾组织中肿瘤坏死因子-α(TNF-α)和白细胞介素-10(IL-10)水平的变化情况.同时用相同剂量生理盐水腹腔注射做为对照组.结果 在PQ中毒大鼠血浆、肺脏、肾脏TNF-α水平在72 h内随时间逐渐升高,在120 h下降;大鼠血浆、肺脏、肾脏IL-10水平在120 h内随时间逐渐升高.PQ组血浆、肺脏TNF-α水平在6 h,12 h,24 h,72 h,120 h与对照组同时段相比显著升高(P<0.05);而PQ组肾脏TNF-α水平在12 h,24 h,72 h,120 h显著高于对照组(P<0.05).PQ组血浆、肺脏IL-10水平在12 h,24 h,72 h,120 h与对照组同时段相比明显升高(P<0.05);而肾脏IL-10水平在24 h,72 h,120 h与对照组同时段相比有显著增加(P<0.05).结论 百草枯中毒可以引起肺、肾组织损伤,其损伤程度与TNF-α和IL-10水平变化关系密切,提示这种损伤的发生可能与TNF-α和IL-10参与机体的炎症反应和细胞免疫密切相关.
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