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首页> 中文期刊> 《沈阳农业大学学报》 >双氢青蒿素对犬乳腺肿瘤细胞生物学特性的影响

双氢青蒿素对犬乳腺肿瘤细胞生物学特性的影响

         
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摘要

犬乳腺肿瘤是犬类的一种常见肿瘤性疾病,目前治疗多为手术结合放、化疗法,但结果多易转移复发.双氢青蒿素(dihydroartemisinin,DHA)是中药提取物,近年来其抗肿瘤作用越来越受到重视.为探求双氢青蒿素是否影响犬乳腺肿瘤细胞的侵袭及其影响机制,本试验取对数增长期状态相同的犬乳腺肿瘤细胞CHMm,使用低(5μM)、中(10μM)、高(20μM)浓度DHA处理并分别培养24,48,72h.收集各组细胞,使用CCK-8法检测细胞活性;利用细胞划痕试验检测细胞迁移性;利用transwell检测细胞侵袭性;利用CCK-8检测细胞毒性.结果表明:DHA对CHMm细胞抑制率呈明显药物浓度依赖性.细胞划痕试验检测细胞迁移速率结果表明,相同培养时间,与空白对照组相比,低、中、高试验组对CHMm细胞迁移性抑制作用效果极显著(p<0.01).各组组间比较,DHA对细胞迁移性抑制影响差异极显著(p<0.01).DHA对CHMm细胞迁移性抑制呈明显的药物时间、浓度依赖性.细胞侵袭试验检测细胞侵袭性结果表明,相同培养时间,与空白对照组相比,低、中、高试验组穿透基底膜着色细胞个数显著增多,说明DHA对CHMm细胞侵袭性抑制效果极显著(p<0.01),组间比较,不同DHA作用浓度效果差异显著(p<0.05).相同DHA作用浓度,随着培养时间的增加,细胞侵袭性降低,效果显著(p<0.05).细胞毒性结果表明,相同培养时间,与空白对照组相比,低、中、高试验组对CHMm细胞抑制性影响效果极显著(p<0.01).各组组间比较,DHA对细胞抑制性影响差异极显著(p<0.01).综上所述,DHA对犬乳腺肿瘤细胞增殖、迁移和侵袭作用具有显著抑制作用,且随着DHA作用浓度增加和时间增长,抑制作用增强,呈浓度时间依赖性.%Canine breast tumor is one of the most serious diseases that threaten health of canine. At present, most of the treatment for breast cancer is surgery, radiotherapy and chemotherapy, but the results are more prone to relapse. As a traditional Chinese medicine extract, dihydroartemisinin (DHA) has been gradually revealed for its anti-tumor effect. This study was conducted to investigate the effects of DHA on the invasion of canine breast tumor cells. The CHMm cells at the logarithmic growth phase, under low (5 M), medium (10 M), high (20M) concentration of DHA treatments were cultured for 24, 48, 72 hours, respectively. Cells were collected and stained by CCK-8. Cell scratch test was made for cell migration. The invasion detection was made by transwell cells. The cytotoxicity of CHMm cells was detected by CCK-8. Compared with the blank control group, the low, medium and high test group had significant inhibition (p<0.01). Compared with the other groups, the effect of DHA on cell inhibition was very significant (p<0.01). The inhibition rate of DHA on CHMm cells was significantly dependent on drug concentration. The cell migration rate of cell scratch test showed that, in the same incubation time, compared with the blank control group, the low, medium and high test group had significant inhibitory effect on the migration of CHMm cells (p<0.01). Compared with the other groups, the effect of DHA on cell migration inhibition was very significant (p<0.01). Compared with theother groups, the effect of DHA on cell migration inhibition was very significant. Compared with the control group, low and high in the experimental group through the basement membrane staining cell number increased significantly, indicating that DHA on CHMm cells had invasion inhibition effect significantly (p<0.01). The effects of different concentrations of DHA were significantly different between groups (p<0.05). In the same concentration of DHA, with the increase of culture time, the invasion of the cells decreased, and the effect was significant (p<0.05). DHA inhibited the migration and invasion of CHMm cells in a time and concentration dependent manner.

著录项

  • 来源
    《沈阳农业大学学报》 |2017年第5期|611-616|共6页
  • 作者

    董婧; 郭昕; 杨淑华; THERESA Hickey; 程荣杰; 张雅楠; 陈月蕾; 李林; WAYNE Tilley;

  • 作者单位

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    阿德莱德大学Dame Roma Mitchel肿瘤研究实验室;

    阿德莱德5000;

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    沈阳农业大学畜牧兽医学院;

    沈阳110161;

    阿德莱德大学Dame Roma Mitchel肿瘤研究实验室;

    阿德莱德5000;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 TP391.41;TP274.52;
  • 关键词

    双氢青蒿素; 犬; 乳腺肿瘤; 侵袭性;

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