[Objective] To explore the inter and intraspecifically physiological and biochemical character-istics of three barrenwort species and to provide theoretical basis of standardized cultivation with high yield.[Method] The morphology, the relative content of chlorophyll (SPAD value) and stomatal conduct-ance of three transplanted barrenwort species were investigated .Non-parametric test and one-way ANO-VA were used to analyze the data .[Result and conclusion] The order of plant height with young leaves from high to low was Epimedium wushanese Ying, Epimedium pubescen Maxim, Epimedium acuminatum Franch .And there were significant differences between E.acuminatum and the other two plants .The or-der of plant height with old leaves from high to low was E.wushanese, E.acuminatum, E.pubescen. And there were significant differences between E.pubescen and the other two plants .Significant differ-ences of leaf morphology of plants with old leaves were observed among three species .But no significant differences was observed among plants with young leaves of three species .For three species , the chloro-phyll contents of all plants with old leaves were higher than those of plants with young leaves .Significant differences of stomatal conductance were observed among three intraspecifically species with young leaves were slightly higher than those of old leaves .The different barrenwort species had different degress of a-daptability to the growth environment .%[目的]揭示不同淫羊藿属Epimediam物种之间及种内的生理生化特征差异，为人工规范化栽培种植及产量提高提供理论参考．[方法]测量了3种移栽淫羊藿属植物的植株高度、叶形态、叶绿素相对含量和气孔导度等生物指标，并运用非参数检验和邓肯氏单因素方差分析对数据进行了比较分析．[结果和结论]新叶植株高度：巫山淫羊藿E．wushanese＞柔毛淫羊藿E．pubescen＞粗毛淫羊藿E．acuminatum，其中，巫山淫羊藿与柔毛淫羊藿、粗毛淫羊藿植株高度均差异显著．老叶植株高度：巫山淫羊藿＞粗毛淫羊藿＞柔毛淫羊藿，其中，柔毛淫羊藿与巫山淫羊藿、粗毛淫羊藿植株高度均达显著差异．3个种间老叶植株的叶片形态差异显著，而新叶不显著；种间及种内的老叶和新叶的叶绿素相对含量均具有显著差异，且老叶植株的叶绿素相对含量均大于新叶植株．气孔导度的种内差异显著，新叶植株叶片的气孔导度大于老叶植株叶片的气孔导度．说明不同淫羊藿属植物对生长环境的适应能力不同．
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