Objective To explore the effects of silencing HERC4 on the proliferation,apoptosis,and migration of cervical cancer cell line Hela and the possible molecular mechanisms.Methods Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells,and HERC4 expression in the cells was examined with Western blotting.CCK-8 assay,annexin V-FITC/PI assay,and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation,apoptosis and migration ability of Hela cells.The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.Results Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P＜0.01).HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells,increased the apoptosis rate (P＜0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P＜0.01).Conclusion Silencing of HERC4 efficiently inhibits the proliferation,migration,and invasion of Hela cells in vitro,and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.%目的 探讨HERC4对宫颈癌细胞生物学功能的影响及其分子机制.方法 设计特异HERC4siRNA干扰序列,Lipofectamine2000法转染Hela细胞,Western blotting检测转染特异siRNA后Hela细胞HERC4蛋白的表达水平;CCK-8法检测转染后Hela细胞的增殖能力;Annexin V-FITC/PI双染法检测转染后Hela细胞凋亡率的变化;划痕实验检测转染后Hela细胞迁移能力.Western blotting检测转染后Hela细胞CyclinD1、Bcl-2表达变化.结果 转染特异siRNA后Hela细胞HERC4蛋白表达量明显下降,差异有统计学意义(P＜0.01).与对照组相比,干扰组Hela细胞生长速度明显减慢,细胞凋亡率增加,迁移能力明显下降,差异有统计学意义(P＜0.01).转染后Cyclin D1和Bcl-2在蛋白水平的表达显著下调(P＜0.01).结论 siRNA可有效降低宫颈癌Hela细胞中HERC4的表达,并通过抑制Cyclin D1表达抑制宫颈癌Hela细胞的增殖和迁移能力,抑制Bcl-2表达增加细胞凋亡能力.
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