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首页> 中文期刊> 《南方医科大学学报》 >核因子I-C降低皮肤成纤维细胞对TGF-β的敏感性

核因子I-C降低皮肤成纤维细胞对TGF-β的敏感性

         
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目的：探讨核因子I-C（NFI-C）对血小板源性生长因子（PDGF）促进皮肤成纤维细胞表达TGF-β受体Ⅱ（TβRⅡ）作用的影响。方法含有NFI-C序列的慢病毒转染人皮肤成纤维细胞（HFF-1）；根据细胞生长活性及转染效率筛选最佳转染复数（MOI）；PDGF-BB刺激体外培养的HFF-1细胞、转染NFI-C的HFF-1细胞以及转染阴性病毒的HFF-1细胞，并设立转染NFI-C但不用PDGF处理的细胞；以不做任何处理的HFF-1细胞作为空白对照组；采用western blot、RT-qPCR测定各组细胞TβRⅡ的表达。数据采用单因素方差分析、LSD-t及SNK-q检验对各组数据进行分析。结果慢病毒转染HFF-1最适MOI为50。PDGF处理的HFF-1细胞表达TβRⅡ高于空白对照组（P<0.05）；在PDGF作用下，转染NFI-C的HFF-1细胞表达TβRⅡ低于未转染的细胞（P<0.05）；阴性病毒无明显抑制作用（P>0.05）；单纯转染NFI-C的HFF-1细胞表达TβRⅡ与空白对照组无显著性差异（P>0.05）。结论 NFI-C能抑制PDGF对TβRⅡ的上调作用，降低皮肤成纤维细胞对TGF-β的敏感性。%Objective To investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-βreceptor II (TβRII) in dermal fibroblasts. Methods A lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RT-qPCR were used to detect the expression levels of TβRII in the treated cells. Results PDGF treatment significantly increased the expression level of TβRII in HFF-1 cells (P<0.05). The cells transfected with Lenti-GFP-NFI-C expressed a significantly lower level of TβRII than non-transfected cells in response to PDGF stimulation (P<0.05), but the negative virus showed no such inhibitory effect (P>0.05). No significant difference was found in the expression level of TβRII protein between cells transfected with Lenti-GFP-NFI-C-transfection before PDGF stimulation and the blank control cells. Conclusion NFI-C can inhibit PDGF-induced up-regulation of TβRII and thus reduce the sensitivity of the dermal fibroblasts to TGF-β.

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