植物细胞工程技术已在农作物的品种改良和培育中得到广泛应用。本文应用细胞培养技术与常规育种方法进行水稻种质创新选育的研究，利用花粉培养建立了水稻垩白性状的近等基因系，选育出了水稻新品系“津稻294”；通过离体胚培养进行水稻杂交种及突变系的胚挽救，并成功获得了野生水稻与栽培稻杂交后代的永久营养体。%Plant cell engineering is extensively used in breeding and variety improvement of crops. Cell culture and crossbreeding were used to breed new germplasm of rice. Near-isogenic line on grain chalkiness was constructed by pollen culture, and had bred the new line“Jindao 294”. The hybrids and mutation of rice were cultured by using embryo rescue and remedy, and the permanent trophosome of hybridization progeny of wild rice and cultivated rice was obtained.
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