Objective: To construct the recombinant adenovirus carrying S100A9 gene, and to explore its ef-fect on the proliferation and apoptosis of squamous cervical cancer cells. Methods: The shuttle plasmid pHBAd-MCMV-RFP-S100A9 was constructed and co-transfected into HEK293 cells with backbone plasmid pHBAd-BHG to produce the recombinant adenovirus Ad-S100A9. Then the C-33A cells were infected with Ad-S100A9, cells infected with Ad-RFP as negative control group, and cells without infection as blank group. S100A9 protein expression was examined by western blot. CCK-8 and flow cytometry were carried out to examine the effects of S100A9 over-expression cell proliferation and apoptosis of C-33A cells, respectively. Results: Ad-S100A9 was successfully constructed. Ad-S100A9 infection significantly enhanced the expression of S100A9 in C-33A cells, and effectively promoted cell proliferation (P<0.05), but had no effect on cell apoptosis. Conclusion:Ad-S100A9 can mediate S100A9 over-expression and promote cell proliferation of squamous cervical cancer cells.%目的:构建携带S100A9基因的重组腺病毒,并研究其对宫颈鳞癌细胞增殖和凋亡的影响.方法:构建穿梭质粒pHBAd-MCMV-RFP-S100A9,与骨架质粒pHBAd-BHG共转染HEK293细胞,获得重组腺病毒Ad-S100A9,Ad-S100A9转染人宫颈鳞癌细胞C-33A作为转染组,以转染Ad-RFP的C-33A细胞作为阴性对照组,未行转染的C-33A细胞为未转染组.采用Western blot法检测S100A9蛋白的表达,使用CCK-8法和流式细胞术,分别研究Ad-S100A9介导S100A9高表达对C-33A细胞增殖与凋亡的影响.结果:成功构建重组腺病毒Ad-S100A9,Ad-S100A9转染C-33A细胞导致S100A9高表达.转染组细胞的增殖能力明显强于未转染组与阴性对照组(P<0.05),转染组细胞的凋亡率与未转染组及阴性对照组比差异无统计学意义(P>0.05).结论:Ad-S100A9重组腺病毒可介导S100A9高表达,S100A9主要通过促进癌细胞的增殖参与宫颈鳞癌的发生发展.
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