目的：研究WNK1激酶对Maxi K通道的调节作用。方法：将携带WNK1和Maxi K目的基因的质粒DNA转染在HEK-293T细胞上，分别应用细胞免疫荧光染色、Western blot观察WNK1对Maxi K在细胞上分布及总蛋白表达水平的影响。结果：免疫荧光染色发现实验组的Maxi K在细胞上的分布明显增多。Western blot结果显示，与对照组相比，实验组1和实验组2的Maxi K总蛋白表达水平均明显升高（P＜0.01）；与实验组1相比，实验组2的Maxi K总蛋白表达水平亦明显升高（P＜0.05）。结论：WNK1能上调Maxi K的总蛋白表达水平，且随着WNK1 DNA剂量的增加，上调作用逐渐增强，显示其上调作用具有剂量依赖性。%Objective: To investigate the adjusting effect of WNK1 kinase on Maxi K channel in HEK-293T cells.Methods: The DNA plasmid carrying WNK1 and Maxi K gene was transfected into the HEK-293T cells, to observe the distribution and total protein expression of Maxi K in the HEK-293T cells via immunolfuo-rescence microscopy and Western blot.Results: Immunolfuorescence microscopic study showed that Maxi K expression was markedly increased in experimental group compared with the control group. Western blotting analysis showed that total Maxi K protein level was markedly increased in the presence of experimental group 1 and experimental group 2 compared with the control group (P<0.01). The total Maxi K protein level was marked-ly increased in experimental group 2 compared with the experimental group 1 (P<0.05).Conclusion: WNK1 can upregulate total protein expression of Maxi K, and the effect is enhanced with the increasing amount of WNK1 DNA, showing that the effect was dose-dependent.
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