Objective To investigate the effects of chronic sodium depletion induced by low sodium diet on salty taste function using conditioned taste aversion (CTA)-two-bottle choice test. Methods Rats fed with normal or low sodium diet were both divided into conditioned and unconditioned groups with 10 in each group. After CTA to 0. 1 mol/L NaCl was established, a series of 30 min two-bottle choice tests were performed with NaCl solutions and distilled water in two bottles. Preference scores for each NaCl solution were calculated. Salty taste detection threshold is defined as the lowest concentration at which there is a reliable difference in the preference scores between conditioned and control subjects. Results The salty taste detection threshold of normal rats was 0. 003-0.005 mol/L NaCl while the detection threshold of rats fed with 14-day low sodium diet decreased to 0.001 - 0.003 mol/L. Conclusion Chronic sodium depletion induced by low sodium diet decreases the salty taste detection threshold, thus increasing rats' sensitivity to salty taste.%目的 采用条件性厌味(conditioned taste aversion,CTA)——双瓶选择方法检测低钠饮食性缺钠对咸味觉感受功能的影响.方法 正常饮食大鼠及低钠饮食大鼠均分为条件组和非条件组,每组10只.建立实验大鼠对0.1mol/L NaCl溶液CTA后,给予系列浓度的NaCl和蒸馏水30 min的双瓶选择测试,计算各溶液的味觉喜好值.根据条件组大鼠喜好值显著低于非条件组喜好值的最低味觉溶液浓度,确定咸味觉检测阈值.结果 正常大鼠咸味觉检测阈值为0.003～0.005 mol/L NaCl,而低钠饮食14d后该阈值降至0.001～0.003 mol/L.结论 慢性脱钠可下调咸味觉检测阈值,使大鼠对咸味刺激更加敏感.
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