＜正＞This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was obtained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 ℃, which is higher than that of free acylase I （60 ℃）. Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.
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机译：Modification of cellulose acetate membrane via low-pressure plasma polymerization for sugar separation applications: Part I. Membrane development and characterization
机译：Electrochemical Characterization of Cellulose Acetate Membranes. 2. Influence of Casting Conditions and of Thermal Curing of CA-Membranes on the EMF of Concentration Cells with HCl, LiCl, NaCl and KCl Solutions
机译：Electrochemical Characterization of Cellulose Acetate Membranes. 1. Influence of Hydrogen and Calcium Ions on the EMF of LiCl Concentration Cells with a CA-Membrane as Separator
机译：(Journal of Chromatography B,840(2):076-084)purification of Vp3 protein of infectious bursal disease virus using nickel ion-immobilized regenerated cellulose-based membranes
