A method of high performance liquid chromatography (HPLC) was established for quantification of L-cysteine based on charge transfer reaction. L-cysteine reacts with tetrachloroquinone in pH 9. 0 borax-boric acid buffer at 40 ℃ for 30 min, forming a complex (A, = 358 nm), which could be detected using HPLC. The results indicated that stable complex formed with an L-cysteine/tetrachloroquinone molar ratio of 1:1. Moreover, the determination fell into an excellent linear relation in the range of 10～150 μg/mL. The recoveries were 99. 2%, 99. 1% and 96. 5% , and the relative standard deviations (RSD) were 1. 3%, 1. 4% and 1. 3% for low, middle and high three kinds added, respectively. This method is accurate, quick, simple and reproducible and can be used to quantify L-cysteine in crude drug.%建立基于荷移反应的L-半胱氨酸高效液相色谱(HPLC)定量方法.在pH9.0的硼酸-硼砂缓冲溶液中,L-半胱氨酸与四氯苯醌于40℃水浴中恒温30 min,形成络合物(λmax=358 nm),采用高效液相色谱进行分析.结果表明:L-半胱氨酸与四氯苯醌形成了摩尔比为1∶1型稳定络合物.另外,L-半胱氨酸在5～100 μg/mL质量浓度范围内具有良好的线性关系；低、中、高3个质量浓度的加样回收率分别为99.2％,99.1％和96.5％,相对标准偏差分别为1.3％,1.4％和1.3％.本方法准确、快速、简便且重复性良好,适用于L-半胱氨酸原料药的定量分析.
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