The optimal conditions of purification of total flavonoids from Ardisia mamillata with macro- porous resin were studied, i.e. the absorption and desorption properties of four kinds of macroporous resin. Results showed that AB-8 macroporous resin had the best adsorption capacity under following technic condi- tions： the initial concentration, flow velouty, pH value and desovption flow velouty were at 0. 34 mg/mL, 2. 0 mL/min, 5.0 and 1.50 mL/min, respectively, with 3BV 70% ethanol as washiny solution. The purity of flavonoids was from 8.92% to 67.79%, which was 7.6 times higher than that of the original sample.%对大孔吸附树脂富集纯化虎舌红（Ardisia mamillata）总黄酮的最佳工艺参数进行了研究，并比较了4种大孔树脂对虎舌红总黄酮的吸附与解吸效果，从中筛选出适合分离纯化虎舌红总黄酮的树脂，同时对其吸附和解吸条件进行了探讨．结果表明，AB-8型为较理想树脂，其纯化总黄酮的最优工艺条件为：上样液质量浓度为0．34mg／mL，吸附流速为2．0mL／min，吸附pH值为5．0，洗脱剂为3BV（90mL）体积分数70％的乙醇，解吸流速为1．50mL／min．经AB-8型树脂纯化后，总黄酮纯度由原来的8．92％提高到67．79％，提高了7．6倍．
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