目的 观察β-咔啉类衍生物(HMD)对人肝癌裸小鼠皮下移植瘤生长的影响.方法 雄性BALB/c裸小鼠每只以2×107个活细胞/ml(0.2 ml)建立人肝癌的裸小鼠模型,随机分为HMD低剂量组(0.22 mg/kg); HMD中剂量组(0.44 mg/kg); HMD高剂量组(0.87 mg/kg);5-氟尿嘧啶注射液组(5-Fu)和生理盐水对照组.各组连续给药15d.观察HMD对裸小鼠人肝癌移植瘤生长的影响及对裸小鼠主要脏器的毒性.结果 HMD低、中、高剂量组及阳性组对裸小鼠人肝癌移植瘤体重的影响与阴性对照组比较均有极显著性差异(P＜0.01).HMD中、高剂量组及阳性组对裸小鼠体质量增加影响与阴性对照组比较有极显著性差异(P＜0.01).对肝脏重量及肝脏系数的影响,HMD低、中、高剂量组及阳性组与阴性对照组比较有显著性差异(P＜0.05),肾脏及肾脏系数各组间差异不显著(P＞0.05).结论 HMD对人肝癌HepG2裸小鼠皮下移植瘤生长有明显的抑制作用,未见有异常毒性症状出现.%Objective To investigate the effect of (a)-carboline derivative HMD on subcutaneous transplant hepatocellular carcinoma in BALB/c nude mice.Methods A tumor model of human HCC was created by subcutaneous inoculation of 2 × 107 (cells/ml,0.2ml) HepG2 cells into BALB/c male nude mice.The mice were randomized to low-(0.22 mg/kg),medium-(0.44 mg/kg),and high-dose HMD (0.87 mg/kg)group,saline group and 5-Fu group.The mice in each group received iv injection of the drug for 15 days.The effects of HMD on the growth of human hepatocellular carcinoma,the weight and pathological changes of nude mice￡§S main organs were observed.Results Compared with saline group,the mice in HMD groups and 5-Fu group showed significant inhibition in the growth of the transplanted tumor (P＜0.01).The inhibitory rate of HMD on the mouse weight.Compared to the saline group,the inhibitory effects of HMD(0.44,0.87 mg/kg) were of statistic significance (P＜0.01).Contrast to the negative control,there were no significant difference to the weight of kidney and kidney index in HMD groups and 5-Fu group,but there were significant differeces in the weight of liver and liver index (P＜0.05).Conclusion HMD exhibit an inhibitory effect on the growth of transplanted HepG2 tumor,and the mice don't appear toxicity symptom.
展开▼
