基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）具有快速、高通量、高准确性和低成本等特点，为临床微生物的鉴定带来了革命性的变化，也给细菌耐药性分析提供了一条新思路。常规细菌耐药性检测方法有纸片法、稀释法、 E-test等，这些方法均需要长时间的培养，不能满足临床对快速药物敏感性指导的需求。MALDI-TOF MS用于细菌耐药性的检测具有巨大的应用价值与前景。已有一些关于MALDI-TOF MS在细菌耐药性检测的实验和临床应用的报道。文章就近年来MALDI-TOF MS在细菌耐药性分析相关的病原体种类、检测方法和检测效果等方面作一综述。%The application of matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) has brought a revolutionary change for microbial identification program in clinical microbiological laboratories,for its fast,high-throughput,accuracy and cost-effectiveness. Furthermore,MALDI-TOF MS provides a new way for drug resistance determination. It is time-consuming for commonly-used methods of routine drug resistance determination,such as Kirby-Bauer,dilution and E-test. These methods can not meet the clinical demand for quick selection of sensitive drugs. So,the application of MALDI-TOF MS for the determination of drug resistance hasresearch value and prospect. Some experiments and clinical application of MALDI-TOF MS for determining drug resistance have been reported. This review introduces the types of pathogens,determination methods and the efficiency of MALDI-TOF MS for determining drug resistance in recent years.
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