目的 证实类风湿因子(RF)对酶联免疫吸附试验(ELISA)检测乙型肝炎e抗原(HBeAg)假阴性干扰的存在.方法 收集乙型肝炎表面抗原(HBsAg)、HBeAg均阴性而RF阳性新鲜血清样本,与HBeAg阳性对照品9:1混合配成HBeAg阳性且RF阳性的试验样本,分别用不同的ELISA试剂检测样本的HBeAg.使用RF吸附乳胶对干扰样本进行预处理,比较预处理前后HBeAg水平的变化.结果 试验中RF对3种不同厂商生产的HBeAg ELISA试剂均有不同程度的假阴性干扰.9例假阴性样本预处理后有7例转为阳性.结论RF可影响ELISA检测HBeAg的S/CO值,引起假阴性结果,样本预处理可以有效消除大部分RF的干扰.%ObjectiveTo investigate whether rheumatoid factor(RF) can cause false negative interference on serum hepatitis B e antigen(HBeAg)by enzyme-linked immunosorbent assay(ELISA).MethodsSerum samples with negative hepatitis B surface antigen(HBsAg),negative HBeAg and positive RF were collected and comprised by blending HBeAg-positive and RF-positive control substance at a ratio of 9:1. HBeAg level was determined by different ELISA kits. RF adsorption latex was used to remove RF from samples,and the change of HBeAg level was compared before and after pre-treatment.ResultsThe 3 kinds of HBeAg ELISA kits had false negative interference. There were 7 of 9 false negative samples transforming to positive after pre-treatment. ConclusionsThe S/CO of HBeAg determination by ELISA is susceptible to falsenegative interference from RF. The pre-treatment of samples is effective prior to the interpretation of determination results.
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