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首页> 中文期刊> 《检验医学》 >结核分枝杆菌动物建模试验中ABSL-3实验室内生物安全评估

结核分枝杆菌动物建模试验中ABSL-3实验室内生物安全评估

         
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目的 通过在动物生物安全三级(ABSL-3)实验室内对结核分枝杆菌(MTB)动物建模过程采样监测,为实验室生物安全评估提供依据.方法 在经中国合格评定国家认可委员会(CNAS)认可并由卫生部批准的,可从事MTB等高致病性病原微生物实验动物研究工作的ABSL-3实验室内实施MTB动物建模,在实验过程中进行空气和物体表面采样.用快速分子诊断和常规病原学方法对样本进行特异性检测分析.结果 生物安全三级(BSL-3)实验室使用后的清场工作可以大大减少实验室环境的细菌量,BSL-3实验室在日常使用中也会存在少量的环境微生物,由于负压强度和使用频率的差异,核心区的环境洁净度明显高于准备间.70％乙醇喷洒消毒及擦拭可以使准备间物体表面的细菌量检出率从59.26％下降到9.38％；核心区物体表面的环境细菌检出率从6.59％下降到1.52％,核心区MTB检出率从10.99％下降到0.在ABSL-3实验室和生物安全柜双重负压环境下,可避免空气中MTB气溶胶污染.结论 ABSL-3实验室的负压环境和正确的防护及消毒措施对实验室操作人员的生物安全有重要的防护作用.%Objective To monitor the sampling process of animal biosafety level 3 ( ABSL-3 ) lab during Mycobacterium tuberculosis ( MTB) animal model construction experiments, and provide basis for biosafety evaluation of ABSL-3 lab. Methods During the process of MTB model construction, air samples and facility surface samples were collected from the authorized ABSL-3 lab by China National Accreditation Service for Conformity Assessment (CNAS). The rapid molecule technology and traditional pathogenic method were used in samples' specificity detection. Results General environmental microbes could be tested in biosafety level 3 ( BSL-3 ) lab, but the core room was cleaner than preparing room most probably because of the negative air pressure and high cleaning frequency. The 70% alcohol can reduce general bacterium detection rate from 59. 26% to 9. 38% , from 6. 59% to 1. 52% in preparing room and from 10. 99% to 0 in core room. The MTB aerosol contamination was not detected in ABSL-3 and biohazard safety equipment under dual negative pressure. Conclusions The appropriate negative air pressure and proper use of biosafety cabinet sterilization measures could help to avoid MTB contamination and ensure the biosafety of ABSL-3 lab.

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