为了对人标准血清总蛋白浓度进行定量测定,提出了一种以蓝紫光半导体激光器为激励光源、以四羧基金属酞菁锌为光谱探针,采用共振瑞利散射法检测蛋白质的新方法.该方法是将人标准血清总蛋白和酞菁锌溶液相结合,当激光光源照射到样品池中的混合液时,将激发出强烈的共振瑞利散射荧光信号,根据信号强度的强弱,计算血清蛋白浓度的大小.结果表明,由于采用了辐射波长位于最佳激励波长范围内的单色辐射光源(405 nm)的蓝紫光半导体激光器作为激励光源,可在90°的接收方向接收到475nm的散射增强光；根据数字示波器显示的探测器散射信号,可得出电压的峰峰值,其体现了荧光的大小,从而可以进一步计算出血清蛋白的浓度.%Using a blue purple semiconductor laser as an excitation light source and using metal phthalocyanine as a spectral probe, a new method to detect protein resonance light scattering was proposed to measure the standard serum protein concentration of human quantitatively. After mixing the phthalocyanine solution with human standard serum protein together, strong fluorescence light of resonance Rayleigh scattering was excited from the mixture. The concentration of serum protein was calculated according to the intensity of the fluorescence signal. From the test result, a semiconductor laser of 405nm blue purple light was selected as an excitation light sources whose radiation wavelengths was fall in the best excitation wavelength range, the 475nm scattering enhanced light can be received in the direction of perpendicular to the direction of input laser light. In the experiment, the peak-to-peak voltage of a digital oscilloscope shows the intensity of the fluorescence light and we calculate the concentration of serum protein after calibration.
展开▼
