Surface plasmon resonance（SPR）is a phenomenon which occurs when light is reflected off thin metal films,and attenuation strength of reflected light depends on incidence wavelength,incidence angle,and refractive index of the material on the surface of Au film.Based on this phenomenon,some SPR bisensors have been developed and been extensively used for characterizing and quantifying biomolecular interactions.In this paper,the working principles,technical parameters and testing method for SPR biosensors are discussed.The concentrations of chicken essence,sugar solution and salt solution tested by HPSPR-6000,are described.%表面等离子共振（Surface Plasmon Resonance,SPR）是一种入射光照引起金属表面产生等离子体共振而导致反射光强衰减的现象,反射光衰减程度与入射光波长、入射角及金属表面物质的折射率相关。根据这原理研究制的表面等离子共振光生物传感器,在检测、分析分子间的反应作用等方面有着广泛的应用前景。本文对表面等离子共振传感器的工作原理、技术参数、检测方法进行了详细的介绍,并用HPSPR-6000型表面等离子共振分析仪对鸡精、糖水及盐水的浓度进行了检测分析。
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