首页> 中文期刊> 《光:科学与应用(英文版)》 >Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes

         

摘要

A donut excitation moves around a single molecule with a zigzag configuration lattice by lattice.Such a method implemented in scanning fluorescence microscopy simplifies the conventional MINFLUX process.Consisting of hollow zero-intensity excitation,single-pixel detection,time-correlated single photon counting,and drift stabilization,the system achieves localization precision and resolution very close to conventional MINFLUX theoretically and experimentally.An averaged high-SNR reference,and pixel-registered intensity from a single molecule is essential to reconstruct localization in maximum likelihood estimation.With performance reaching nearly conventional MINFLUX's,the proposed raster-scanning MINFLUX can inspire researchers expertized in STED or confocal setup to quickly transform to MINFLUX and develop for further exploring on bio-specimens or optical applications.

著录项

  • 来源
    《光:科学与应用(英文版)》 |2022年第10期|2271-2275|共5页
  • 作者

    Xinzhu Xu; Shu Jia; Peng Xi;

  • 作者单位

    Department of Biomedical Engineering;

    College of Future Technology;

    Peking University;

    Beijing 100871;

    China;

    Wallace H.Coulter Department of Biomedical Engineering;

    Georgia Institute of Technology and Emory University;

    Atlanta;

    Georgia 30332;

    USA;

    UTS-SUStech Joint Research Centre for Bio-medical Materials&Devices;

    Department of Biomedical Engineering;

    College of Engineering;

    Southern University of Science and Technology;

    Shenzhen;

    Guangdong 518055;

    China;

    National Biomedical Imaging Center;

    Peking University;

    Beijing 100871;

    China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 光电子技术、激光技术;
  • 关键词

    scanning; excitation; stabilization;

相似文献

  • 中文文献
  • 外文文献
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号