Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

Yue Zhuo; Ji Sun Choi; Thibault Marin; Hojeong Yu; Brendan A.Harley; Brian T.Cunningham

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Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

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Focal adhesions are critical cell membrane components that regulate adhesion and migration and have cluster dimensions that correlate closely with adhesion engagement and migration speed.We utilized a label-free approach for dynamic,long-term,quantitative imaging of cell–surface interactions called photonic resonator outcoupler microscopy(PROM)in which membrane-associated protein aggregates outcoupled photons from the resonant evanescent field of a photonic crystal biosensor,resulting in a highly localized reduction of the reflected light intensity.By mapping the changes in the resonant reflected peak intensity from the biosensor surface,we demonstrate the ability of PROM to detect focal adhesion dimensions.Similar spatial distributions can be observed between PROM images and fluorescence-labeled images of focal adhesion areas in dental epithelial stem cells.In particular,we demonstrate that cell–surface contacts and focal adhesion formation can be imaged by two orthogonal label-free modalities in PROM simultaneously,providing a general-purpose tool for kinetic,high axial-resolution monitoring of cell interactions with basement membranes.

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《光：科学与应用（英文版）》 |2018年第1期|949-963|共15页
作者
Yue Zhuo; Ji Sun Choi; Thibault Marin; Hojeong Yu; Brendan A.Harley; Brian T.Cunningham;
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作者单位

Department of Bioengineering;

University of Illinois at Urbana-Champaign;

Urbana;

IL 61801;

USA;

Micro and Nanotechnology Laboratory;

University of Illinois at Urbana-Champaign;

Urbana;

IL 61801;

USA;

Department of Chemical and Biomolecular Engineering;

University of Illinois at Urbana-Champaign;

Urbana;

IL 61801;

USA;

Carl R.Woese Institute for Genomic Biology;

University of Illinois at Urbana-Champaign;

Urbana;

IL 61801;

USA;

Atkins Building;

University of Illinois Research Park;

1800 South Oak Street;

Champaign;

IL 61820;

USA;

Department of Electrical and Computer Engineering;

University of Illinois at Urbana-Champaign;

Urbana;

IL 61801;

USA;

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原文格式 PDF
正文语种 chi
中图分类妇产科学;
关键词
PROM; reflected; resonator;

Quantitative analysis of focal adhesion dynamics using photonic resonator outcoupler microscopy (PROM)

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