为查明波纹巴非蛤(Paphia undulata)幼贝最适生长条件,采用响应面法研究了温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响。利用Box-Behnken中心组合进行3因素3水平的实验设计(3因素为温度(A),盐度(B),密度(C)；3水平为-1,0,1),以幼贝生长率为响应值,以A(26℃,28℃,30℃)、B(28,30,32)和C(0.56,1.4,2.24个/cm2)为影响因子。实验结果表明：(1)盐度对幼贝生长影响最显著(P<0.05),3种生态因子对幼贝生长率影响程度大小顺序是：B>A>C；(2)通过SAS软件分析,得出波纹巴非蛤幼贝最适生长环境组合是温度为26.93℃、盐度为31.39、密度为1.12个/cm2,理论最佳生长率为41.47%。为验证响应面法预测结果的准确性,以预测结果为实验条件,相同实验时间后,得到的生长率为42.30%,结果与理论预测值相近,响应面方法可靠。%In order to explore the optimum growth conditions for Paphia undulata youth, response surface method-ology was used to study the effect of temperature, salinity and density on growth of Paphia undulata youth. The experiment was designed according to Box-Behnken central composite design in three factors and three levels, and temperature (26℃, 28℃, 30℃), salinity (28, 30, 32), density (0.56,1.4,2.24ind/cm2) were selected as affecting fac-tors. The results showed that the effect of salinity on the growth rate was the most significant (P <0.05), and the order of three ecological factors effects on the youth growth was salinity (B) > temperature (A) > density (C). By means of SAS software, the optimum growth environment condition of Paphia undulata youth was confirmed as follows: temperature 26.93℃, salinity 31.39 and density 1.12ind/cm2, and theoretical optimal growth rate was 41.47%. After experimental verification, the growth rate was 42.30%. To verify the accuracy of the results predicted by RSM, the real growth rate was 42.30%under the theoretical condition, suggesting RSM was reliable.
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