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4个缢蛏群体遗传多样性和系统发生关系的微卫星分析

         

摘要

采用12个微卫星分子标记,对4个不同缢蛏(Sinonovacula Constricta)群体:福建云霄群体、广东湛江群体、天津塘沽群体以及浙江乐清湾群体进行了遗传多样性和系统发生关系的分析。研究结果显示,12个微卫星共有46个等位基因,4个缢蛏群体的平均观测等位基因数(Ao)、平均观测杂合度(Ho)、平均期望杂合度(He)和平均多态信息含量(PIC)分别为3.25~3.50、0.59~0.74、0.55~0.58和0.46~0.50。说明4个缢蛏群体遗传多样性处于中等多态。UPGMA 聚类分析表明:乐清湾群体、天津塘沽群体间的遗传距离最小,亲缘关系最近,首先聚为一支,福建云霄群体和广东湛江群体聚为另一支。群体的 F-统计量(Fst)为0.07,表明缢蛏群体分化很微弱。%Twelve microsatellite markers were used to study genetic diversity and phylogenetic relationship of four geographic populations of razor clam Sinonovacula constricta. The four populations were sampled from Yunxiao City of Fujian, Zhanjiang City of Guangdong, Tanggu City of Tianjin and Yueqing City of Zhejiang. The results showed that total of 46 alleles were found among 12 SSR markers. The average observed number of alleles (Ao) was from 3.25 to 3.50, the average observed heterozygosity (Ho) was from 0.59 to 0.74, the average excepted het-erozygosity (He) was from 0.55 to 0.58 and the average polymorphism information content (PIC) ranged from 0.46 to 0.50, respectively. All of these results indicated that genetic diversity of the four populations was at a medium level. UPGMA cluster analysis showed that Yueqing population was clustered with Tanggu population into one clad, then Yunxiao population and Zhanjiang population were in a second group. The value of the F-statistics was 0.07 and this suggested that the four populations of S.Constricta had a relatively lower genetic variation and its genetic structure between populations was weak.

著录项

  • 来源
    《海洋科学》 |2013年第8期|96-102|共7页
  • 作者单位

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

    浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室,浙江 温州,325001;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 Q959.2;
  • 关键词

    缢蛏(Sinonovacula Constricta); 微卫星; 遗传多样性; 遗传距离;

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