Hydroxyapatite (HAp)/carboxymethyl chitosan (CMCS) composite scaffolds cross linked with genipin were prepared by particle filtration and lyophilization.The tissue engineering bone was produced by compounding HAp/CMCS scaffolds with pcDNA3.1-vascular endothelial growth factor (VEGF) plasmid-transfected bone marrow stromal cells (BMSCs),and the repair effect on bone defects was evaluated by the osteogenic activity in rabbit radial defect model.The microstructure and mechanical property of genipin crosslinked HAp/CMCS scaffold are similar to that of natural cancellous bone,which could meet the requirements of the scaffold materials.Comparing to glutaric dialdehyde crosslinked scaffold,the genipin crosslinked scaffold has less toxicity and more facility for adhesion and proliferation of cell,additional to its spontaneous fluorescence features.Animal experiments showed the bone is a potential material for bone defect repairing which has a good biocompatibility,nontoxicity,microcirculation promotion and acceleration of bone defect repair and the degrading rate could match that of bone growing.%以京尼平(Genipin)为交联剂,通过粒子沥滤结合冷冻干燥工艺制备纳米羟基磷灰石/羧甲基壳聚糖复合支架.然后依据组织工程的原则,将pcDNA3.1-血管内皮生长因子(Vascular endothelial growth factor,VEGF)165质粒转染后的骨髓间充质干细胞(Bone marrow stromal cells,BMSCs)与支架复合构建组织工程骨.最后将该组织工程骨植入到兔桡侧,观察其成骨能力及降解速度.结果表明:京尼平交联的支架材料的微观结构、力学性能与天然松质骨相似,可满足支架材料的要求；且支架材料具有自发荧光特征,便于观察支架的微结构及界面上细胞的粘附情况；动物实验表明所得复合骨具有生物相容性好、无毒副作用的优点及促进局部微血管形成、加快骨缺损修复的作用,其降解速度与骨生长速度基本匹配,是一种潜在的性能优良的骨修复材料.
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