目的：构建含PRDX5基因启动子的荧光素酶报告质粒。方法①通过生物信息学方法，查询PRDX5基因启动子核酸序列；②使用PCR扩增人PRDX5基因启动子片段，将启动子插入到pGL3-Basic载体中，构建含PRDX5基因启动子片段的荧光素酶报告质粒；③双酶切及测序确定 PRDX5基因启动子的荧光素酶报告质粒扩增正确。结果 PCR扩增的PRDX5启动子片段插入到载体中，经测序证实正确。结论成功构建含PRDX5启动子的荧光素酶报告质粒。%Objective To construct a pGL3-PRDX5-promoter-Luc plasmid and to vertifying its luciferase activity. Methods ①search the nucleic acid sequence of the promoter PRDX5 by bioinformatics analysis.②Methods of PCR was performed to amplify the promoter PRDX5. the promoter PRDX5 was inserted into pGL3-Basic cloning vector to construct luciferase reporter plasmid with the promoter PRDX5.③pGL3-PRDX5-promoter-Luc plasmid was verified by double-enzyme cleavage and sequencing method.Results pGL3-PRDX5-promoter-Luc plasmid was identified to be correct by double-enzyme cleavage and sequencing method.Conclusion A pGL3-PRDX5-promoter-Luc plasmid was successful y constructed.
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