目的：探讨RhoA及微血管密度(MVD)与宫颈癌发生发展的关系。方法采用免疫组织化学SP法,分别测定16例正常宫颈组织、20例宫颈上皮内瘤变组织、50例宫颈鳞癌组织中RhoA蛋白及CD34的表达。结果①宫颈鳞癌组RhoA的阳性表达与宫颈上皮内瘤变组和正常宫颈组比较有明显差异(<0.001；<0.001)。②宫颈鳞癌组MVD值与宫颈上皮内瘤变组和正常宫颈组比较有明显差异(<0.001；<0.001)。③Ⅱ期宫颈鳞癌RhoA和MVD的阳性表达分别与I期比较,差异有统计学意义(<0.05)。组织学分级G3 RhoA和MVD的阳性表达分别与G1RhoA和MVD比较有明显差异(<0.05)。宫颈鳞癌淋巴结转移组RhoA的阳性表达与无淋巴结转移组比较,无明显差异(>0.05),MVD比较也无差异(>0.05)。<35岁的年轻宫颈癌患者中RhoA的阳性表达率与≥35岁的宫颈癌患者比较,差异有统计学意义(<0.001)。两组患者MVD值比较,差异无统计学意义(>0.05)。结论在宫颈癌发生发展中,RhoA的表达增强和MVD增高可能发挥了重要作用。%Objective To estimate the expressions of RhoA and microvessel density (MVD)in squamous carcinoma of the cervix (SCC), cervical intraepithelial neoplasia (CIN) and normal cervical epithelium (NCE) and evaluate the possible mechanism in the invasion of cervical carcinoma. Methods In 16 NCE cases,20 CIN cases and 50 SCC cases,immunohistochemical S-P method had been used to examine the expression levels of RhoA and CD34. Results ①The expression level of RhoA was significantly higher in SCC than in CIN and NCE ( <0.001, <0.001). ② The MVD was significantly higher in SCC than in CIN and NCE ( <0.001, <0.001). ③ In comparison to stageⅠSCC, the expression levels of RhoA and MVD in stageIIwere significant difference ( <0.01;<0.01). In comparison to G1 SCC, the MVD and the expression levels of RhoA in G3 had significant dif erence ( <0.05;<0.001). In comparison to the without lymph nodal metastasis group, the expression levels of RhoA and MVD in SCC with lymph node metastasis group was no significantly different ( >0.05;>0.05). The expression levels of RhoA in more than 35 years old was higher than in less than 35 years old, there was significant dif erence( <0.001), but MVD was no significantly different in the two groups( >0.05). Conculusion RhoA might be a key role in the invasion and metastasis of squamous carcinoma of the cervix. The increase of microvessel density is closely related to the progression of tumor. Their levels maybe act as clinical referential indexes in the progression of cervical carcinoma.
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