目的 通过使用香烟烟雾提取物(CSE)刺激人气道上皮细胞株细胞,检测MUC5AC mRNA的表达,并观察血栓素A2受体拮抗剂SQ29548对NCI-H292细胞MUC5AC mRNA表达的干预作用.方法 采用细胞培养法,分别探讨CSE诱导人气道上皮细胞MUC5AC mRNA表达的浓度依赖效应和CSE诱导NCI-H292细胞MUC5AC mRNA表达的时间依赖效应.检测细胞中MUC5AC mRNA的含量以及SQ29548对NCI.H292细胞MUC5AC mRNA表达的影响.结果 不同浓度CSE(1%,2%,4%,5%)均可上调MUCSAC的mRNA表达.各时间点实验组与对照组相比,MUC5AC mRNA表达均高于对照组.SQ29548可以抑制MUC5AC mRNA的基础表达,且这种抑制作用呈荆量依赖性.结论 香烟烟雾提取物能诱导NC卜H292细胞MUC5ACmRNA的表达增加,这种诱导作用具有时间和浓度依赖效应.TXA2受体拮抗荆SQ29548能显著抑制CSE诱导的NCI-H292细胞MUC5ACmRNA的表达增加.%Objective To research the influence of thromboxane A2 receptor antagonist, SQ29548, on expression of MUC5AC of human airway epithelium induced by cigarettes smog extract (CSE). Methods The human airway epithelium was stimulated with cigarettes smog extract. The expression of MUC5AC mRNA was detected. Results CSE could up-regulate the expression of MUC5AC mRNA and SQ29548 could inhibit the expression of MUC5AC mRNA. Conclusion CSE can induce MUC5AC mRNA expression, which can be attenuated by hromboxane A2 receptor antagonist, SQ29548.
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