Construction of plant expression vector of adketo gene driven by a tomato fruit-special promoter

Xin-Jun Chen; Jing Huang; Wei Li; Ke-Yan Zhong; Xin-Zheng Gao

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Construction of plant expression vector of adketo gene driven by a tomato fruit-special promoter

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Objective:To construct the plant expression vector including Adketo gene from Adonis aestivalis and E8 promotor, and provide a new way for astaxanthin production by plant genetic engineering.Methods: The Adketo gene was clonied from Adonis aestivalis by RT-PCR and the 1.1 kb tomato E8 gene was amplified by PCR. then cloned into T vector.After the preliminary identification by Microbial PCR, the recombinant T- vectors were subjected to sequence analysis. Cut the E8 promoter and Adketo gene and inserted into the plant binary expression vector pBI121to obtain the recombinant expression vector. And then to identify the vector by microbial PCR detection and enzyme digestion.Results: Sequencing results analysis showed the right E8 and Adketo gene sequences, the identification by microbial PCR and digestion with restriction enzymes proved that the recombinant vector had the inserts with expected length of target fragments.Conclusion: The plant expression vector containing Adketo gene driven by fruit specific promoter E8 was successfully constructed.

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《海南医科大学学报(英文版)》 |2019年第2期|P.11-14|共4页
作者
Xin-Jun Chen; Jing Huang; Wei Li; Ke-Yan Zhong; Xin-Zheng Gao;
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作者单位

[1]School of Basic Medical Science;

Hainan Medical University;

Haikou 571199;

China;

[1]School of Basic Medical Science;

Hainan Medical University;

Haikou 571199;

China;

[1]School of Basic Medical Science;

Hainan Medical University;

Haikou 571199;

China;

[1]School of Basic Medical Science;

Hainan Medical University;

Haikou 571199;

China;

[1]School of Basic Medical Science;

Hainan Medical University;

Haikou 571199;

China;

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原文格式 PDF
正文语种 CHI
中图分类肿瘤学;
关键词
Adketo gene; Expression vector; E8 promoter;

机译：Adketo基因;表达载体;E8启动子;

Construction of plant expression vector of adketo gene driven by a tomato fruit-special promoter

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