Objective:To prepare the liposomes of mangrove oil,Optimization of the formulation of mangrove oil liposomes by Box Behnken response surface methodology.Methods:Preparation of Rhododendron oil liposomes by ethanol injection probe ultrasound,Determination of gemacrone by HPLC.The ratio of lecithin to cholesterol(X1),drug lipid ratio(X2)and phospholipid concentration(X3)were used as independent variables,and encapsulation efficiency(Y)was used as dependent variable,the formulation was optimized by Box Behnken response surface method,and the entrapment efficiency was predicted.The entrapment efficiency,particle size,polydispersity index(PDI),Zeta potential and drug loading of the optimized liposomes were evaluated.Results:The optimal prescription and preparation of Folium Rhododendri Daurici oil liposome was confirmed as follows:X1=7.28:1、X2=11.34:1、X3=9.32mg·mL-1,the encapsulation efficiency was(82.55±1.66)%,the particle size was(130.531±46)nm,the polydispersity index was 0.185±05,Zeta potential was(21.970±36)mV,the drug loading was(5.941±0.12)%.Conclusion:The Box Behnken response surface method is accurate to obtain the optimal formulation of mangrove oil liposomes,it has high precision and good prediction effect.And the preparation process of mangrove oil liposomes is stable and feasible.
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机译：Optimization of Medium Composition for Antibacterial Substance Production by Aspergillus niger xj using Response Surface MethodologyAU Li, Zhu (zhuliluck@163.com) Liu, Song Song, Yuan Liu, Wu-Juan Chen, Xue Wang, Qiang Ge, Yong-Yi Shi, Miao-Xiao Jin, Yi
