Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

Zheng-Dong Guo; Yang-Yang Bian; Xiao-Qian Liu; Dong Wang; Si-Yuan Zhang; Jian Yang; Lei Peng

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Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

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Objective:To investigate the inhibitory effect of calcium hydroxide on methicillin-resistant Staphylococcus aureus and the related inhibition mechanism.Methods:To determine the minimum inhibitory concentration of calcium hydroxide using microplate dilution method;to compare the effects of calcium hydroxide at 8MIC,MIC,1/4MIC and 0 concentrations on MRSA using growth curve method;to determine the effects of calcium hydroxide on the cell membrane of methicillin-resistant Staphylococcus aureus using calcium xanthophyll and propidium iodide fluorescence staining The effect of calcium hydroxide on the morphology of methicillin-resistant Staphylococcus aureus was observed by scanning electron microscopy;the inhibition mechanism of calcium hydroxide on MRSA was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis of protein bands.Results:The MIC of calcium hydroxide on MRSA was 3.125 mg/mL;the fluorescence intensity showed significant changes after co-culture of calcium hydroxide with bacteria;MRSA appeared to be significantly crumpled and broken in the presence of calcium hydroxide;the SDS-PAGE experimental bands indicated that the protein inside the bacteria decreased accordingly with the increase of calcium hydroxide concentration.Conclusion:Calcium hydroxide has a significant inhibitory effect on the growth of MRSA,and its bactericidal mechanism may be related to the destruction of bacterial body structure.

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《海南医科大学学报(英文版)》 |2022年第5期|18-22|共5页
作者
Zheng-Dong Guo; Yang-Yang Bian; Xiao-Qian Liu; Dong Wang; Si-Yuan Zhang; Jian Yang; Lei Peng;
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作者单位

Trauma medical center of the First Affiliated Hospital of Hainan Medical College 570216;

Key Laboratory of Emergency and Trauma Ministry of Education Medical University 570216;

Key Laboratory of Hainan Trauma and Disaster Rescue First Affiliated Hospital of Hainan Medical University 570216;

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原文格式 PDF
正文语种 chi
中图分类无机化学;
关键词
Calcium hydroxide; Methicillin-resistant staphylococcus; MRSA; BACTERIOSTASIS; Antibacterial mechanism; Cytomembrane;

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Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus

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