Screening of Anti-tumor Effective Extracts from Sedum bulbiferum Makino and HPLC Determination of Quercetin and Kaempferol in This Herbal Medicine

Liming MENG; Xiangluan WAN; Luyang LI; Jing HU; Pu CHEN; Dingrong WAN

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Screening of Anti-tumor Effective Extracts from Sedum bulbiferum Makino and HPLC Determination of Quercetin and Kaempferol in This Herbal Medicine

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[Objectives] To investigate the anti-tumor activity of Sedum bulbiferum Makino in vitro,and establish a HPLC method for determination of quercetin and kaempferol in S. bulbiferum. [Methods] The inhibitory activities of different extracts and total flavonoids of S. bulbiferum on proliferation of three kinds of cancer cells( Hep G2,EC109,SW480) were tested by MTT assay. HPLC method for determination of quercetin and kaempferol in S. bulbiferum was established. [Results]The growth and proliferation of the three kinds of cancer cells were all significantly inhibited by ethyl acetate fraction and total flavonoids isolated from S. bulbiferum. With each extraction concentration increasing,stronger anti-tumor activity was found. The linear ranges of quercetin and kaempferol were 0. 03-0. 36 μg( R = 0. 999 9) and0. 08-0. 96 μg( R = 0. 999 9),and the average recoveries were 98. 90%( RSD = 1. 15%) and 98. 27%( RSD = 1. 70%),respectively.[Conclusions]S. bulbiferum has significant anti-tumor activity in vitro. The HPLC method established was accurate,reproducible,and could be used for quality control of this crude drug.

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《药用植物：英文版》 |2019年第3期|P.30-33|共4页
作者
Liming MENG; Xiangluan WAN; Luyang LI; Jing HU; Pu CHEN; Dingrong WAN;
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作者单位

[1]Shiyan Institute for Food and Drug Control;

Shiyan442000;

China;

[2]School of Foreign Languages;

Hubei University of Chinese Medicine;

Wuhan430071;

China;

[3]College of Pharmacy;

South-central University for Nationalities;

Wuhan430074;

China;

[3]College of Pharmacy;

South-central University for Nationalities;

Wuhan430074;

China;

[3]College of Pharmacy;

South-central University for Nationalities;

Wuhan430074;

China;

[3]College of Pharmacy;

South-central University for Nationalities;

Wuhan430074;

China;

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原文格式 PDF
正文语种 CHI
中图分类中药化学;
关键词
Sedum bulbiferum Makino; Anti-tumor activity; Quercetin; Kaempferol; Content determination;

机译：景天景天;抗肿瘤活性;槲皮素;山emp酚;含量测定;

Screening of Anti-tumor Effective Extracts from Sedum bulbiferum Makino and HPLC Determination of Quercetin and Kaempferol in This Herbal Medicine

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