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首页> 外文期刊>药用植物：英文版 >Extraction Process Optimization and Anti-oxidant Activity of Total Flavonoids in Dendrobium nobile Lindl.
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Extraction Process Optimization and Anti-oxidant Activity of Total Flavonoids in Dendrobium nobile Lindl.

机译：石D中总黄酮的提取工艺优化及总黄酮的抗氧化活性

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摘要

[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fraction,the ultrasonic-assisted extraction process of total flavonoids from D.nobile Lindl.was optimized using quadratic general rotary unitized design,and the in-vitro anti-oxidant activity of the flavonoids extracted was evaluated preliminarily.[Results]The optimized extraction process for flavonoids in D.nobile Lindl.was as follows:extraction time 10 min,solid/liquid ratio 1∶15(g∶mL),extraction temperature 75℃and ethanol volume fraction 90%.According to the revised optimal process,6 repeated experiments were carried out,and the average yield of flavonoids was(0.5515±0.0004)mg/g,consistent with the predicted value of the model.The results of anti-oxidation experiment show that the flavonoids extracted from D.nobile Lindl.have better scavenging effect on ABTS and DPPH free radicals,and the scavenging activity is proportional to the mass concentration of the extract.[Conclusions]The optimized extraction process for flavonoids from D.nobile Lindl.is reasonable and feasible,and the flavonoids in D.nobile Lindl.have strong antioxidant activity.
机译：[目标]以提高石斛中石斛黄酮的产量。[方法]基于4个固体/液体比的单因素实验，提取温度，提取时间和乙醇体积分数，超声波辅助提取过程总计来自D.Nobile Lindl.was的黄酮，采用二次通用旋转整体设计优化，提取的黄酮类化合物的体外抗氧化活性进行预先评价。[结果] D.Nobile Lindl.was中黄酮类化合物的优化提取方法遵循：提取时间10分钟，固体/液体比1:15（G:ML），提取温度75℃和乙醇体积分数90％。根据修订的最佳过程，进行了6个重复的实验，以及平均产量黄酮类化合物（0.5515±0.0004）mg / g，与模型的预测值一致。抗氧化实验结果表明，从D.Nobile Lindl中提取的黄酮类化合物。在ABTS和DPPH免费RA上具有更好的清除效果直接的活性和清除活性与提取物的质量浓度成比例。[结论]来自D.Nobile Lindl的黄酮类化合物的优化提取方法合理可行，具有D.Nobile Lindl中的黄酮类化合物。有力的抗氧化活性。

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