[Objective] To study changes of piglet's jejunum pathology and jejunum flora caused by porcine circovirus 2 type (PCV2) infections.[Methods] The viremia of three piglets infected with PCV2 by oral and intramuscular injection was determined.Then six weaned pigs,being randomly divided into PCV2 infected group and the control group (n=3),were infected with PCV2 with the same route of infection.The three piglets of each group were slaughtered at 21 d after infection.Jejunum and its contents were collected for microscopic pathology and flora sequencing.[Results] The virus loads of the piglets' serum achieved a higher level at 21 d after PCV2 infection.The jejunum villous of piglets in the infection group atrophied and fell off.The microbial species and abundance in these jejunums changed,and jejunum flora diversity increased,including the significant decrease of Lactobacillus content and the significant increase of Pseudomonas genus content.[Conclusion] PCV2 infection results in certain changes in piglet's jejunum flora.A decline in the jejunum beneficial bacteria and the increase of harmful bacteria may further aggravate the piglet jejunum inflammation.%[目的]研究猪圆环病毒2型(PCV2)感染导致的仔猪空肠病理变化及其菌群变化.[方法]经过口服和肌肉注射PCV2后测定3头仔猪病毒血症情况;随后将6头断奶仔猪随机分为感染组和对照组,感染组3头,空白组3头.相同接种途径感染仔猪,在感染后21 d宰杀仔猪,无菌采集空肠肠段及内容物分别进行显微病理观察和MiSeq测序分析空肠菌群变化.[结果]PCV2感染后21 d仔猪血清中病毒核酸达到较高水平,感染组仔猪空肠绒毛萎缩脱落,空肠微生物种类和丰度发生变化,菌群多样性增高,乳酸菌属含量显著降低,假单胞菌属含量显著增高.[结论]PCV2感染导致仔猪空肠菌群发生一定变化,空肠有益菌减少,有害菌增多,加重仔猪空肠炎症发生.
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