通过构建16S rDNA克隆文库,对承德地区两温泉中的细菌多样性水平及系统发育关系进行了初步研究.研究表明:68℃的A11文库中阳性克隆的16S rDNA序列分属5个细菌类群,分别为 Firmicutes (6.25％)、Deinococcus-Thermus (25.0％)、Gammaproteobacteria (12.5％)、Betaproteobacteria (50.0％)、Alphaproteobacteria(6.25％)；而74.5℃的A12文库仅属于一个细菌类群:厚壁菌门(Firmicutes).两温泉中细菌多样性的差异表明,温度是影响温泉中细菌多样性水平的重要因素.此外,A11文库中克隆的16S rDNA序列与许多已知的可产色素的好氧菌相似性很高,而A12文库中的细菌多数为专性厌氧或兼性厌氧型,其中厌氧芽孢杆菌属(Anoxybacillus)中的Anoxybacillus flavithermus可以作为研究泉华形成的理想材料.%The bacterial diversity and phylogenetic analysis of two hot springs in Chengde were investigated and analyzed by construction of 16S rRNA gene clone libraries. The results showed that bacteria in sample All (68℃) could be divided into 5 groups, which were as follows: Firmicutes (6.25%), Deinococcus-Thermus (25.0%), Gammaproteobacteria (12.5%), Betaproteo-bacteria (50.0%), Al-phaproteobacteria (6.25%). But sample A12 (74.5℃) contains only one group, Firmicutes. The difference between the two samples revealed that the temperature was an important factor to affect the level of bacterial diversity in hot springs. Furthermore, a lot of 16S rDNA sequences in Al 1 clone library had high similarity to the aerobic bacteria that can produce pigment, while most bacteria in A12 clone library belonged to obligate or facultative anaerobe, in which, Anoxybacillus flavithermus can be used as ideal material to research the formation of sinter.
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