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桑粒肩天牛肠道纤维素分解细菌的分离和鉴定

         

摘要

为研究天牛消化利用纤维素的机理,利用纤维素-刚果红琼脂培养基,从桑粒肩天牛(A.germari)幼虫中肠分离到一种兼性厌氧纤维素分解菌。菌落为白色圆形,边缘比较规则,周围溶纤维素透明圈直径一般可达10mm~20mm,细菌大小为0.5~0.8μm×1~3μm,革兰氏染色阳性,杆状,极生鞭毛,无芽孢。结合生化实验结果,初步鉴定该菌株为纤维单孢菌属(Cellulomonas)。%In order to clarify the cellulose-utilizing mechanism of the Cerambycidae, the microorganism in the Apriona germari(Hope) larvae's gut was cultured and identified. A strain of facultative anaerobic cellulose-utilizing bacteria was isolated from the midgut fluid of A. germari larvae with the cellulose-congo red agar medium. The colonies of the bacteria were white and round with regular margin. Clear zones of cellulolysis surrounding the colonies might reach 10m~20mm. The size of the bacteria was 0.5μm~0. 8μm× l~3μm approximately. The cell was gram-positive rod with polar flagella. It hadn't spores. With the results of biochemical tests, the bacteria were tentatively identified as Cellulomonas.

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