A strain C4 with ability of bioconversion from steroid RSA to hydrocortisone was selected fron the soil sample. It was identifide as Cuvularia Iunata according its morphological characters of clump and individuals. The optimum culture medium was established by orthogonal experiments :glucose 10g ,soybean powder 10g,water 1000mL, pH6.5. Determined by silica gel chromatography and high-performance liquid chromatography, the bioconversion ratio of hydrocortisone was 34%. At the same tine, the percentage change of RSA,hydrocortisone and other byproducts were measured during the course of bioconversion.%从土样中筛选到1株能转化甾体化合物RSA(17α-羟基孕甾-4-烯-3，20-二酮-21-醋酸酯)生成氢可的松(11β，17a，2l-三羟基孕甾-4-烯-3，20-二酮)的C4菌株，经菌落形态与个体形态观察，初步鉴定为新月弯胞霉(curvularia lunata)。正交实验确定该菌株的最适培养基为：葡萄糖10g，黄豆粉10g，自来水1000mL，pH6.5.经硅胶层析和高交液相色谱测定，氢化可的松转化率为34％。同时还测定了转化过程中RSA、氢化可的松及其它副产物的百分含量的变化。
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