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形态受培养温度影响的绿脓杆菌菌株

         

摘要

A strain of P. Aeruginosa,which was seperated from clinical environment ,shows a special characteristic . It keeps normal short rod shape when cultured at 37℃,however,it forms filament without pyocyanin producing when cultured at 25℃ overnight. The filaments will divide and form short rods,simultaneously,produce pyocyanin when culture time is prolonged to over 72h or culture temperature is raised to 37℃. The preliminary study indicates that this phenomenin has nothing to do with nutritive conditions and could the inbluenced by inoculating density and irradiating with ultraviolet rays The absence of pyocyanin was not the cause of filamentous formation by the test results.%从临床分离的一株绿脓杆菌表现出特殊性状,该菌在37℃培养时保持正常的短杆状,在25℃培养过夜则形成丝状形态,且不产生绿脓色素。当延长培养时间至72h以上或提高培养温度至37℃,则丝状体开始逐渐断裂形成正常的短杆状菌体,并出现绿脓色素。初步研究表明,该现象与营养条件无关,菌群的生长密度可以影响这一现象,紫外线的照射有促进作用,同时绿脓色素的缺失不是丝状体形成的原因。

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