Aiming at the problem of how to get rid of gas hosting cleanly,an experimental analysis system for degradation of coal and adsorption of methane by methanotrophs in simulated row coal occurrence environment has been developed. A type of facultative anaerobic methanotrophs was selected by the experiment. Three variables were designed by single factor variable method. The effects of temperature, injection pressure and volume on the degradation efficiency of the methanotrophs were investigated.The results showed that when the temperature was 30℃,the injection pressure was 2 MPa and the injection volume was 20 mL,the degradation rate of methane was up to 34.2%;when the temperature was 30 ℃ and the injection volume was 20 mL,the degradation rate of methane increased with the increase of the injection pressure,the rate was up to 45.6%;when the temperature was 30 ℃,the injection pressure was 2 MPa,the degradation rate of methane increased with the increase of the injection volume,the rate was up to 54.6%.%针对如何清洁高效地治理煤矿瓦斯问题,自行研制了模拟原煤体赋存环境下微生物降解煤吸附甲烷实验系统.通过实验培养筛选出一种兼性厌氧型甲烷氧化菌,采用单因素变量法,共设计3个水平,实验考察了环境温度、菌液注入压力和体积对该甲烷氧化菌的降解效能的影响.实验结果表明:该厌氧型甲烷氧化菌种在最适温度30 ℃,菌液注入压力2 MPa、注入体积20 mL时,甲烷降解率最高可达到34.2%;在温度30℃、菌液注入体积20 mL时,甲烷降解率随着菌液注入压力增大而增大,甲烷降解率最高为45.6%;在温度30 ℃、菌液注入压力2 MPa时,甲烷降解率随着菌液注入体积的增加而增大,甲烷降解率最高为54.6%.
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