Objective:To establish an HPLC method for determination of monosaccharide content of polysaccharides inTremella fuciformis,and compare the difference of monosaccharide content in Tremella from different sources.Methods:The optimized method of the acid hydrolysis,and PMP precolumn derivative HPLC method were applied to analyze the monosaccharide composition of 12 batches Tremella cultivated from woods log in Tongjiang and 8 batch ones cultivated from artificial media(cottonseed hull) in Fujian.Results:The experiments results showed that the optimized method was appropriate,and there were significant differences in the content of mannose,glucose,fructose in different Tremella samples.Conclusion:There exist a significant differences in composition of Tremella polysaccharide of different sources and cultivating way of Tremella.The study provides reference for the establishment of the Tremella quality standard.%目的:建立HPLC法测定银耳中银耳多糖单糖含量的方法,并比较不同来源银耳中单糖含量的差异.方法:根据银耳特性,优化酸水解-PMP柱前衍生方法以及HPLC色谱条件,对12批次通江段木培育银耳样品以及8批次福建棉籽壳代料银耳进行多糖的单糖组成分析.结果:优化后的实验方法适用于银耳多糖中单糖组分的检测.结论:通过数据对比分析可以看出,不同来源和培育方式的银耳在甘露糖、葡萄糖、岩藻糖等含量上存在着比较明显的差异;通过论证认为,这些差异可以为制定银耳质量标准提供必要的参考和借鉴.
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