Objective:To study alkaloids in stem of Macleaya cordata.Methods:The methanol extract of M.cordata stem was analyzed with High Performance Liquid Chromatography-Quardrupole /Time of Flight-Mass spectrometry,alkaloids was identified by the molecular ions of mass spectrometry and the fragment ions of MS /MS spectrometry combined with mass fragmentation rules of isoquinoline alkaloids.Results: 11 alkaloids were elucidated, which were magnocurarine (1 ), reticuline(2 ),scourlerine (3 ),cyclanoline (4 ),13-methoxyberberine (5 ),tetrahydropalmatine (6 ),protopine (7 ), allocryptopine(8),N-methylcanadine(9),sanguinarine(10),chelerythrine(11),compounds 1-6 and 9 were reported in M.cordata for the first time.Conclusion:The method to identify alkaloids in M.cordata stems based on mass fragmentation rules of isoquinoline alkaloids is feasible.%目的:快速分析博落回茎中的生物碱成分.方法:应用高效液相色谱-四级杆飞行时间质谱对博落回茎的生物碱进行分析,根据一级质谱的分子离子和二级质谱的碎片离子,结合博落回中异喹啉类生物碱的质谱裂解规律,推断博落回茎中的生物碱结构.结果:从博落回茎中推断出11个生物碱的结构,分别为木兰箭毒碱(Magnocurarine,1),网脉番荔枝碱(Reticuline,2),金黄紫堇碱(Scoulerine,3),轮环藤酚碱(Cyclanoline,4),13-甲氧基小檗碱(13-methoxyberberine,5),四氢巴马丁(Tetrahydropalmatine,6),原阿片碱(Protopine,7),别隐品碱(Allocryptopine,8),N-甲基氢化小檗碱(N-methylcanadine,9),血根碱(Sanguinarine,10),白屈菜红碱(Chelerythrine,11),其中化合物1~6及9首次在博落回中报道.结论:经验证,基于异喹啉生物碱质谱裂解规律推断博落回茎中生物碱的方法可行.
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