目的 探讨CD4+CD25+调节性T细胞特异标志物FOXP3在慢性乙型肝炎(CHB)中的作用及其与TGF-β1的相关性.方法 120例CHB患者均进行肝脏活体组织穿刺检查,以炎症活动度分级进行分组.免疫组织化学染色法检测不同炎症活动度分级患者肝组织中FOXP3及TGF-β1的表达变化及定位特点.结果 不同病理分级CHB肝组织中FOXP3与TGF-β1评分均高于正常对照组(G0组)(P均<0.05),随着炎症活动度的逐渐增加,FOXP3 与TGF-β1评分逐渐增强,均与炎症活动度呈正相关(r=0.866,P<0.05;r=0.841,P<0.05).肝组织中FOXP3表达与ALT、HBV DNA定量之间无相关性.结论 肝脏炎症的存在是促进FOXP3分泌表达的必要条件.FOXP3可作为判断乙型肝炎的病情和预后的指标之一.FOXP3与TGF-β1/Smads信号传导可能在肝组织损伤及纤维化进展过程中共同发挥了作用.%Objective It is to approach the role of CD4 CD25 regulatory T cells specific markers of FOXP3 in chronic hepatitis B (CHB) and correlation with TGF - β1. Methods 120 patients with CHB were performed liver biopsy , and divided into 4 groups by inflammation grades. The expression changes and positioning characteristics of FOXP 3 and TGF -Β1 in the liver tissue of patients with inflammation grades and normal group ( Group G0) were detected by Immunohistochemical stai - ningResults The score of liver tissue FOXP3 and TGF - β1 in different pathological grading of CHB were higher than that of normal group (P <0. 05 ). FOXP3 and TGF - β1 score were gradually increased with the degree of inflammatory activity gradually increased and both were positively correlated with the degree of inflammatory activity (r =0. 866,P <0.05; r =0. 841, P <0. 05 ). FOXP3 expression in liver tissue was no correlation between ALT and HBV DNA quantitative . Conclusion The presence of inflammation of the liver is the necessary conditions for the secretion and expression of FOXP 3. FOXP3 can be used as one of the indicators to determine the condition and prognosis of hepatitis B . FOXP3 and TGF - β1 Smads signal transduction maybe play a role together in liver injury and fibrosis progresses .
展开▼
