Carbonylation and Loss-of-Function Analyses of SBPase Reveal Its Metabolic Interface Role in Oxidative Stress, Carbon Assimilation, and Multiple Aspects of Growth and Development in Arabidopsis

Xun-Liang Liu; Hai-Dong Yu; Yuan Guan; Ji-Kai Li; Fang-Qing Guo

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Carbonylation and Loss-of-Function Analyses of SBPase Reveal Its Metabolic Interface Role in Oxidative Stress, Carbon Assimilation, and Multiple Aspects of Growth and Development in Arabidopsis

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Sedoheptulose-1,7-bisphosphatase (SBPase) is a Calvin cycle enzyme and functions in photosynthetic carbon fixation.We found that SBPase was rapidly carbonylated in response to methyl viologen (MV) treatments in detached leaves of Arabidopsis plants.In vitro activity analysis of the purified recombinant SBPase showed that SBPase was carbonylated by hydroxyl radicals,which led to enzyme inactivation in an H2O2 dose-dependent manner.To determine the conformity with carbonylation-caused loss in enzymatic activity in response to stresses,we isolated a loss-of-function mutant sbp,which is deficient in SBPase-dependent carbon assimilation and starch biosynthesis,sbp mutant exhibited a severe growth retardation phenotype,especially for the developmental defects in leaves and flowers where SBPASE is highly expressed.The mutation of SBPASE caused growth retardation mainly through inhibition of cell division and expansion,which can be partially rescued by exogenous application of sucrose.Our findings demonstrate that ROS-induced oxidative damage to SBPase affects growth,development,and chloroplast biogenesis in Arabidopsis through inhibiting carbon assimilation efficiency.The data presented here provide a case study that such inactivation of SBPase caused by carbonyl modification may be a kind of adaptation for plants to restrict the operation of the reductive pentose phosphate pathway under stress conditions.

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《分子植物（英文版）》 |2012年第5期|1082-1099|共18页
作者
Xun-Liang Liu; Hai-Dong Yu; Yuan Guan; Ji-Kai Li; Fang-Qing Guo;
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The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai), Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032,China;

The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai), Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032,China;

The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai), Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032,China;

The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai), Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032,China;

The National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai), Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032,China;

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Carbonylation and Loss-of-Function Analyses of SBPase Reveal Its Metabolic Interface Role in Oxidative Stress, Carbon Assimilation, and Multiple Aspects of Growth and Development in Arabidopsis

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