The Medicago truncatula Sucrose Transporter Family: Characterization and Implication of Key Members in Carbon Partitioning towards Arbuscular Mycorrhizal Fungi

Joan Doidy; Diederik van Tuinen; Olivier Lamotte; Marion Corneillat; Gérard Alcaraz; Daniel Wipf

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The Medicago truncatula Sucrose Transporter Family: Characterization and Implication of Key Members in Carbon Partitioning towards Arbuscular Mycorrhizal Fungi

机译：紫花苜蓿蔗糖转运蛋白家族：碳分配对丛枝菌根真菌的关键成员的表征和意义。

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We identified de novo sucrose transporter (SUT) genes involved in long-distance transport of sucrose from photosynthetic source leaves towards sink organs in the model leguminous species Medicago truncatula.The identification and functional analysis of sugar transporters provide key information on mechanisms that underlie carbon partitioning in plant-microorganism interactions.In that way,full-length sequences of the M.truncatula SUT (MtSUT)family were retrieved and biochemical characterization of MtSUT members was performed by heterologous expression in yeast.The MtSUT family now comprises six genes which distribute among Dicotyledonous clades.MtSUT1-1 and MtSUT4-1 are key members in regard to their expression profiles in source leaves and sink roots and were characterized as functional H+/sucrose transporters.Physiological and molecular responses to phosphorus supply and inoculation by the arbuscular mycorrhizal fungus (AMF) Glomus intraradices was studied by gene expression and sugar quantification analyses.Sucrose represents the main sugar transport form in M.truncatula and the expression profiles of MtSUT1-1,MtSUT2,and MtSUT4-1 highlight a fine-tuning regulation for beneficial sugar fluxes towards the fungal symbiont.Taken together,these results suggest distinct functions for proteins from the SUT1,SUT2,and SUT4 clades in plant and in biotrophic interactions.

机译：我们在模型豆科植物苜蓿三叶草（Medicago truncatula）中鉴定了蔗糖从光合来源叶片向水槽器官的长距离运输中涉及的蔗糖转运蛋白（SUT）基因。糖转运蛋白的鉴定和功能分析提供了构成碳分配基础的关键信息通过这种方法，检索了沙眼衣原体SUT（MtSUT）家族的全长序列，并通过酵母中的异源表达对MtSUT成员进行了生化鉴定.MtSUT家族现在包含六个基因，这些基因分布在双子叶进化枝.MtSUT1-1和MtSUT4-1在源叶和汇根中的表达谱是关键成员，并被表征为功能性H + /蔗糖转运蛋白。对磷供应和丛枝菌根真菌接种的生理和分子响应（ AMF）通过基因表达和糖度研究Glomus内辐射r定量分析。蔗糖代表represents藜中的主要糖转运形式，MtSUT1-1，MtSUT2和MtSUT4-1的表达谱突出显示了有益的糖通向真菌共生体的微调规则。综合考虑，这些结果提示了植物和生物营养相互作用中SUT1，SUT2和SUT4进化枝的蛋白质具有不同的功能。

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《分子植物（英文版）》 |2012年第6期|1346-1358|共13页
作者
Joan Doidy; Diederik van Tuinen; Olivier Lamotte; Marion Corneillat; Gérard Alcaraz; Daniel Wipf;
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UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, P(o)le Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France;

UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, P(o)le Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France;

UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, P(o)le Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France;

UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, P(o)le Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France;

UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, P(o)le Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France;

UMR INRA 1347, Agrosup, Université de Bourgogne, Agroécologie, P(o)le Interactions Plantes Microorganismes ERL CNRS 6300, BP 86510, 21065 Dijon Cedex, France;

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The Medicago truncatula Sucrose Transporter Family: Characterization and Implication of Key Members in Carbon Partitioning towards Arbuscular Mycorrhizal Fungi

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