首页> 中文期刊> 《纳微快报:英文版》 >Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers

Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers

         

摘要

A simple,convenient,and highly sensitive bio-interface for graphene field-effect transistors(GFETs) based on multifunctional nano-denatured bovine serum albumin(nano-dBSA) functionalization was developed to target cancer bio-markers.The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface.The formed nano-d BSA film served as the cross-linker to immobilize monoclonal antibody against car-cinoembryonic antigen(anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS.The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing.The improved GFETbiosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL-1.The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction,indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82×10-10M.The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing,nanomedicine,and drug delivery.

著录项

  • 来源
    《纳微快报:英文版》 |2019年第2期|P.24-36|共13页
  • 作者单位

    [1]State Key Laboratory of Transducer Technology;

    Key Laboratory of Terahertz Solid?State Technology;

    Shanghai Institute of Microsystem and Information Technology;

    Chinese Academy of Sciences;

    Shanghai 200050;

    People’s Republic of China;

    [1]State Key Laboratory of Transducer Technology;

    Key Laboratory of Terahertz Solid?State Technology;

    Shanghai Institute of Microsystem and Information Technology;

    Chinese Academy of Sciences;

    Shanghai 200050;

    People’s Republic of China;

    [2]School of Biomedical Engineering;

    Shanghai Jiao Tong University;

    Shanghai 200240;

    People’s Republic of China;

    [2]School of Biomedical Engineering;

    Shanghai Jiao Tong University;

    Shanghai 200240;

    People’s Republic of China;

    [3]School of Electronic Engineering;

    Bangor University;

    Bangor LL57 1UT;

    UK;

    [2]School of Biomedical Engineering;

    Shanghai Jiao Tong University;

    Shanghai 200240;

    People’s Republic of China;

    [1]State Key Laboratory of Transducer Technology;

    Key Laboratory of Terahertz Solid?State Technology;

    Shanghai Institute of Microsystem and Information Technology;

    Chinese Academy of Sciences;

    Shanghai 200050;

    People’s Republic of China;

    [1]State Key Laboratory of Transducer Technology;

    Key Laboratory of Terahertz Solid?State Technology;

    Shanghai Institute of Microsystem and Information Technology;

    Chinese Academy of Sciences;

    Shanghai 200050;

    People’s Republic of China;

  • 原文格式 PDF
  • 正文语种 CHI
  • 中图分类 TP212.3;
  • 关键词

    Bio-interface; Multifunctional denatured BSA; GFET biosensor; Cancer biomarker;

    机译:生物接口多功能变性BSA GFET生物传感器癌症生物标志物;
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