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首页> 中文期刊> 《天然产物研究与开发》 >中药组方“回乳康”对回乳期奶牛血清PRL、GH和STAT5含量的影响

中药组方“回乳康”对回乳期奶牛血清PRL、GH和STAT5含量的影响

         
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摘要

The aims of this study was to explore the effect of "Hui Ru-kang" prescription on serum PRL,GH and STAT5 levels in dairy cow during milk withdrawal period.80 Healthy cows in late pregnancy that gave 15.42 ± 0.71 kg milk per day were used.The cows were randomly divided into four groups:group A,B,C and D with 20 of them in each group.Dry dairy cows' milk by gradually dry milk method and the day when cows began to dry milk was recorded as 0 d.Group A was a control group,did not feed "Hui Ru-kang".Group B,C and D were fed "Hui Ru-kang" 400,500,600 g respectively at 8:00 am from the 1 d to the day that cows withdrawed milk completely.The cows' venous blood was collected from the tail on the day 0,1,3,5,7,9 and 11.In addition,the daily milk production was noted.ELISA was used to evaluate the expression levels of PRL,GH and STAT5.As the result,the milk production of all 4 groups were on the decline during milk withdrawal period.Group A,B,C and D dried milk on 11,7,5 and 5 d respectively.The expression levels of serum PRL,GH and STAT5 were declining in all four groups and the decline trend of group A was slower than other groups,and the expression levels of GH and STAT5 were raised after milk withdrawal period.The change of PRL,GH,STAT5 expression levels and milk yield in group C were not significantly different between these of group D (P ＞ 0.05).The expression levels of PRL,GH,STAT5 and milk yield showed significant and positive correlation among each other (P ＜ 0.01).During milk withdrawal period,feeding 500 g "Hui Ru-kang" would make dairy cows withdraw milk obviously.%为探究中药组方“回乳康”对回乳期奶牛血清催乳素(PRL)、生长激素(GH)和信号转导和转录激活因子5(STAT5)含量的影响.试验选择规模化奶牛场产奶量为15.42 ±0.71 kg的妊娠后期,即将干乳的健康奶牛80头.随机分为A、B、C和D组,每组20头,采用逐渐干奶法回乳,干乳开始当天记为第0d,A组为对照组,不饲喂“回乳康”,B、C、D组均从回乳开始第1d,每日上午8:00分别饲喂“回乳康”400、500、600 g,回乳完成后停止饲喂.分别采集奶牛回乳期第0、1、3、5、7、9和11d尾静脉血,并记录奶牛单日产奶量,ELISA法检测血清PRL、GH和STAT5含量.结果显示,各组回乳期奶牛产奶量均随时间推移降低,A、B、C和D组回乳时间分别为11、7、5和5d.各组PRL、GH和STAT5含量均呈下降趋势,GH和STAT5含量在回乳期后回升,且A组下降趋势较各中药组慢,C组PRL、GH、STAT5含量及产奶量均分别与D组差异不显著(P＞0.05);回乳期奶牛血清PRL、GH、STAT5含量与产奶量两两间均呈极显著正相关(P＜0.01).综上,饲喂500 g/日“回乳康”即可使奶牛回乳效果达到最佳.

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  • 来源
    《天然产物研究与开发》 |2017年第10期|1766-17721758|共8页
  • 作者

    沈留红; 肖劲邦; 巫晓峰; 姜思汛; 曹随忠; 余树民;

  • 作者单位

    四川农业大学动物医学院动物疫病与人类健康四川省重点实验室四川农业大学动物医学院奶牛疾病研究中心,成都611130;

    四川农业大学动物医学院动物疫病与人类健康四川省重点实验室四川农业大学动物医学院奶牛疾病研究中心,成都611130;

    四川农业大学动物医学院动物疫病与人类健康四川省重点实验室四川农业大学动物医学院奶牛疾病研究中心,成都611130;

    四川农业大学动物医学院动物疫病与人类健康四川省重点实验室四川农业大学动物医学院奶牛疾病研究中心,成都611130;

    四川农业大学动物医学院动物疫病与人类健康四川省重点实验室四川农业大学动物医学院奶牛疾病研究中心,成都611130;

    四川农业大学动物医学院动物疫病与人类健康四川省重点实验室四川农业大学动物医学院奶牛疾病研究中心,成都611130;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 兽医母畜科学、产科学;
  • 关键词

    回乳期奶牛; 中药组方“回乳康”; 催乳素; 生长激素; 信号转导和转录激活因子5;

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