This study investigates the anti-tumor effect of Radix Isatidis polysaccharide (RIP) on tumor bearing mice and its influences on immune function.The impacts of RIP were observed by transplanted tumor and ascites tumor within S180 mice,and cyclophosphamide was used as the positive control.After 12 days of continuous administration,the tumor weight,inhibition rate of tumor growth,spleen and thymus index,splenic lymphocyte transformation function,killing activity of NK cells,the levels of TNF-α,INF-γ,IL-2 in serum were detected.HE staining and cell cycle analysis were performed on the tumor tissue.The ascites tumor mice followed up for the same dosing period were fed normally afterwards,and the time of natural death of each group was recorded.The results showed different doses of RIP can all obviously inhibit the growth of tumor in mice,especially the 100 mg/kg and 50 mg/kg group with the inhibition rates of 35.4％ and 38.5％.The spleen index and thymus index of the transplanted mice in each dose group were significantly higher than those of the control group.It also stimulated the proliferation of splenic lymphocytes,enhanced the killing activity of NK cells,and increased the levels of TNF-α,INF-γ,and IL-2 in serum,the 50 mg/kg group compared with the blank control group was statistically significant (P ＜ 0.01).The analysis of HE staining showed that the tumor necrotic area in each dose group was increased in different proportion.Flow cytometry showed that the proportion of G0/G1 phase cells increased,the proportion of S phase cells decreased,G2/M phase cells did not change significantly.It suggested RIP has anti-tumor effect and can enhance the immune function and prolong the survival time of tumor-bearing mice.%本研究主要探讨板蓝根多糖(RIP)对荷瘤小鼠的抗肿瘤作用及其对免疫功能的影响.通过建立S180小鼠移植瘤和腹水瘤模型,以环磷酰胺(CTX)为阳性对照药,观察RIP对其的影响.连续给药12 d后,测定移植瘤小鼠的瘤重,计算肿瘤生长抑制率和胸腺、脾脏指数;检测脾淋巴细胞的转化功能和NK细胞杀伤活性以及血清中的TNF-α、INF-γ、IL-2水平;并对肿瘤组织进行HE染色和细胞周期分析;对进行相同给药周期的腹水瘤小鼠继续正常饲养,记录各组小鼠自然死亡的时间.结果显示,不同剂量的RIP均可明显抑制小鼠肿瘤的生长,其100 mg/kg和50 mg/kg剂量组的抑瘤率分别为35.4％,38.5％;各剂量组移植瘤小鼠胸腺指数和脾指数与对照组比较有所提高,还能刺激脾淋巴细胞的转化及增强NK细胞的杀伤活性,升高血清中TNF-c、INF-γ和IL-2的含量,其50 mg/kg组与模型对照组相比有统计学差异(P＜0.01).此外,移植瘤组织HE染色观察可见各给药组肿瘤组织坏死面积呈不同比例增大,流式细胞仪检测出给药后G0/G1期细胞比例增加,S期细胞比例降低,G2/M期细胞周期未见明显变化.由此提示,RIP能够增强荷瘤小鼠的免疫功能,对荷瘤小鼠具有抗肿瘤作用,能延长荷瘤小鼠的生存时间.
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