To explore the content and the monosaccharide composition of the polysaccharide in Rosa laevigata Mickx and provide references for the quality control and the pharmaceutical utilization of R.laevigata,the total polysaccharide was obtained by water extraction and alcohol precipitation method.The polysaccharide content was detected by anthrone sulfuric acid colorimetry using the glucose calibration.Then the monosaccharide compositions of the polysaccharide were analyzed by TMS pre-column GC-MS method.The results showed that the yield of total polysaccharide was 29.38％.The polysaccharide was composed by arabinose,rhamnose,xylose,mannose,galactose,glucose,galacturonic acid and fructose,and the contents of glucose,mannose,fructose and galactose were reached to 65.03％,7.36％,16.11％ and 8.87％,respectively,obviously higher than the other 4 monosaccharides in R.laevigata polysaccharide.%探讨金樱子多糖的含量及其单糖组成,为金樱子质量评价及临床用药提供参考依据.本文采用水提-醇沉法获得金樱子多糖,以葡萄糖为标准品,蒽酮-硫酸比色法测定多糖含量;多糖酸解后经三甲基硅烷(TMS)衍生化,以标准品单糖为对照,采用气-质联用法(GC-MS)测定多糖的单糖组成.结果表明,本实验条件下,测得金樱子多糖提取得率为29.38％;金樱子多糖中单糖由阿拉伯糖、鼠李糖、木糖、甘露糖、半乳糖、葡萄糖、果糖组成,其中葡萄糖、甘露糖、果糖和半乳糖在金樱子多糖中占比较大,分别为65.03％、7.36％、16.11％及8.87％.
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